| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Phelps, Anna E (9)
|
MAUN-0 |
32.07 |
30.98 |
-1.09 |
| 2 |
Collins, Ryleigh (9)
|
MAPFY |
34.20 |
32.63 |
-1.57 |
| 3 |
Zerfass, Ava D (9)
|
MAEMAC |
38.35 |
34.35 |
-4.00 |
| 4 |
Tassinari, Marianne E (9)
|
MAPFY |
36.07 |
35.78 |
-0.29 |
| 5 |
Iacobelli, Sophia C (9)
|
MAEMAC |
37.12 |
36.56 |
-0.56 |
| 6 |
Lenett, Carly A (9)
|
MAEMAC |
|
36.81 |
- |
| 7 |
Lin, Simone (9)
|
MAPAAC |
40.97 |
37.37 |
-3.60 |
| 8 |
Piacenti, Lily (9)
|
MAPFY |
43.99 |
37.49 |
-6.50 |
| 9 |
Frank, Alice C (9)
|
MAEMAC |
39.03 |
37.57 |
-1.46 |
| 10 |
Karnhaukhova, Anastasiya (9)
|
MAPJA |
44.08 |
38.85 |
-5.23 |
| 11 |
Settle, Ashley (9)
|
MAUNPA |
|
41.47 |
- |
| 12 |
Wiragh, Kendall J (9)
|
MAEMAC |
45.96 |
45.20 |
-0.76 |
| 13 |
Sadler, Cheyenne N (9)
|
MAUNAA |
37.24 |
50.77 |
13.53 |